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[Identification of markers linked to resistance locus of Marssonina leaf spot in poplars by bulked segregant analysis(BSA)].

Identifieur interne : 004517 ( Main/Exploration ); précédent : 004516; suivant : 004518

[Identification of markers linked to resistance locus of Marssonina leaf spot in poplars by bulked segregant analysis(BSA)].

Auteurs : Bo Zhang [République populaire de Chine] ; Min-Ren Huang ; Qiang Zhuge ; Zheng-Min Han ; Tong-Ming Yin ; Hui-Xin Pan ; Li-Huang Zhu ; Rong-Ling Wu ; Ming-Xiu Wang

Source :

RBID : pubmed:16135446

Abstract

DNA markers linked to resistance locus of Marssonina leaf spot in poplars were found by bulked segregant analysis(BSA). The bulks consisted of individual with a extreme phenotype taken from a population of 91 F1 clones,which is a progeny of Populus deltoides Bartr.cv."Lux"(I-69/55)(Resistance) and P.euramericana cv.I-45(Susceptible). Out of 114 RAPD primers, four markers showed polymorphisms between the resistance-bulk and the susceptible-bulk.By using selective genotype linkage analysis,OPAI17-1550 and OPAI13-900 were found linked to the resistance locus. The genetic distances between the two markers and the resistance locus were 29.9cM and 37.4cM,respectively.

PubMed: 16135446


Affiliations:


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